Data publication platform of Utrecht University

van de Lest, Chris H.A.

Bicarbonate-stimulated membrane reorganization in stallion spermatozoa

2021-10-27T10:36:01.684664 Open - freely retrievable

"Bicarbonate-stimulated membrane reorganization in stallion spermatozoa", published in: Frontiers Cell and Developmental Biology This study aimed to investigate and clarify several aspects of stallion sperm capacitation occurring in the presence of bicarbonate, calcium and albumin. Specifically, re-organization of the plasma membrane after the initiation of sperm capacitation was investigated using lipidomics, cryo-electron microscopy, flow cytometry and complementary biochemical strategies. To this end, phosphatidylserine (PS) and phosphatidylethanolamine exposure in viable sperm was monitored with flow cytometry and PS exposure with live imaging. The current data-package contains the raw data files of the lipidomics analysis of these stallion sperm cells. The data is contained in primary Thermo-Fisher Scientific (Waltham, MA, USA) raw LC/MS-data files. The raw data-files were converted to mz(X)ML files by msConvert (part of ProteoWizard v3.0.913) (also contained in this data-package) and processed with the R package xcms v2.99.3. After deisotoping, annotation of lipids was performed by matching measured MS-1 m/z values with theoretical m/z values as described in Molenaar et al., 2019 (doi:10.1093/gigascience/giz061).

Spermatozoa, capacitation, membrane, lipid, bicarbonate, fertilization, equine

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